Glucose responsive insulin production from human embryonic germ (EG) cell derivatives.

TitleGlucose responsive insulin production from human embryonic germ (EG) cell derivatives.
Publication TypeJournal Article
Year of Publication2007
AuthorsClark GO, Yochem RL, Axelman J, Sheets TP, Kaczorowski DJ, Shamblott MJ
JournalBiochemical and biophysical research communications
Volume356
Issue3
Pagination587-93
Date Published2007 May 11
Abstract

Type 1 diabetes mellitus subjects millions to a daily burden of disease management, life threatening hypoglycemia and long-term complications such as retinopathy, nephropathy, heart disease, and stroke. Cell transplantation therapies providing a glucose-regulated supply of insulin have been implemented clinically, but are limited by safety, efficacy and supply considerations. Stem cells promise a plentiful and flexible source of cells for transplantation therapies. Here, we show that cells derived from human embryonic germ (EG) cells express markers of definitive endoderm, pancreatic and beta-cell development, glucose sensing, and production of mature insulin. These cells integrate functions necessary for glucose responsive regulation of preproinsulin mRNA and expression of insulin C-peptide in vitro. Following transplantation into mice, cells become insulin and C-peptide immunoreactive and produce plasma C-peptide in response to glucose. These findings suggest that EG cell derivatives may eventually serve as a source of insulin producing cells for the treatment of diabetes.

Alternate JournalBiochem. Biophys. Res. Commun.