In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I.

TitleIn vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I.
Publication TypeJournal Article
Year of Publication2013
AuthorsSun X, Li Y, Shi M, Zhang N, Wu G, Li T, Qing L, Zhou C
JournalArchives of virology
Volume158
Issue12
Pagination2611-5
Date Published2013 Dec
Abstract

Symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in plant cells.

DOI10.4056/sigs.3667269
Alternate JournalArch. Virol.