Study on urinary profile of inborn errors of metabolism by 18-crown-6 modified capillary electrophoresis with laser-induced fluorescence detection.

TitleStudy on urinary profile of inborn errors of metabolism by 18-crown-6 modified capillary electrophoresis with laser-induced fluorescence detection.
Publication TypeJournal Article
Year of Publication2013
AuthorsZhang J-B, Li M-J, Li Z, Yan X-J, Yuan J-Q, Dong W-X, Zhang Y, Chu Q-C, Ye J-N
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume929
Pagination102-6
Date Published2013 Jun 15
Abstract

Newborn screening in urine is important for the diagnosis of many inborn errors of metabolism (IEM). Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a major technological advance in screening IEM. It has the advantage of sensitive and simultaneous multiple disease screening with minimal sample requirement. The analytes were derivatized with fluorescein isothiocyanate (FITC) prior to CE-LIF analysis. In urine samples, free amino acids (AAs) were well separated from other coexisting components, exhibiting a linear calibration over the concentration range 0.01-5.0μmol/L with the limits of detection (LODs) ranging from 0.005 to 0.010μmol/L. The relative standard deviations (RSDs) were in the range 0.1-1.0% for peak area, and 0.2-1.0% for migration time, respectively. Under optimized conditions, the method presented here has been successfully used for the simultaneous and sensitive analysis of seven AAs in urine samples of newborn babies, and evaluating the effect of therapy as well.

DOI10.1364/OE.21.010772
Alternate JournalJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.