Size-dependent Adsorption Dynamics, Conformation and Function of Bone Morphogenetic Protein-2 onto Silica Nanoparticles.

TitleSize-dependent Adsorption Dynamics, Conformation and Function of Bone Morphogenetic Protein-2 onto Silica Nanoparticles.
Publication TypeJournal Article
Year of Publication2013
AuthorsLi Z, Zhang W, Li Y, Huang B, Yuan Y, Liu C
JournalBio-medical materials and engineering
Volume23
PaginationS815-20
Date Published2013 Jan 1
Abstract

Effective and efficient immobilization onto matrix is extremely crucial for maximizing the function and lowering the dose of BMP-2 in the field of bone tissue engineering. However, how the surface characteristics of matrix affect the way BMP-2 binds to the support and in turn influence its bioactivity remains poorly understood. In this contribution, with silica nanoparticles (SNPs) in the range of 20-100 nm introduced as models, the nanoscale curvature-mediated adsorption dynamics, conformation, and bioactivity of recombinant human BMP-2 (rhBMP-2) were investigated. The data showed that SNPs bound rapidly to and induced unfolding of rhBMP-2 molecules, which undermined their interactions with the corresponding receptors on cell surface and decreased the bioactivities of adsorbed rhBMP-2. In contrast, rhBMP-2 showed increasingly strong affinity to and lost less secondary structure on the larger SNPs, while better bioactivity was observed on the medium SNP60 surfaces. The results indicate that the size of the SNPs, perhaps because of the contribution of surface curvature, influences the structure and function of the adsorbed BMP-2. This study demonstrates the possibility of simple tailor-made nanoscale curvature to mediate the binding, conformation and bioactivity of BMP-2, allowing fabrication of BMP-2-based bone tissue scaffolds.

DOI10.1371/journal.pone.0076147
Alternate JournalBiomed Mater Eng