Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.

TitleSimplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.
Publication TypeJournal Article
Year of Publication2013
AuthorsLi Y, Shen L, Sun Y, Yuan J, Huang J, Li C, Li S, Luo Y, Qiu H-J
JournalJournal of clinical microbiology
Volume51
Issue8
Pagination2710-2
Date Published2013 Aug
Abstract

The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.

DOI10.1128/JCM.00859-13
Alternate JournalJ. Clin. Microbiol.