Semisynthetic lipidated LC3 protein mediates membrane fusion.

TitleSemisynthetic lipidated LC3 protein mediates membrane fusion.
Publication TypeJournal Article
Year of Publication2013
AuthorsYang A, Li Y, Pantoom S, Triola G, Wu Y-W
JournalChembiochem : a European journal of chemical biology
Volume14
Issue11
Pagination1296-300
Date Published2013 Jul 22
Abstract

All together: Lipidated LC3 has been synthesized by expressed protein ligation. A TEV-cleavable MBP tag was employed to facilitate ligation under folding conditions and to solubilize the lipidated protein. The synthetic LC3-phosphatidylethanolamine (PE) mediates membrane tethering and fusion at the physiological concentration of PE, and could be a useful tool for autophagy studies.

DOI10.4056/sigs.3667269
Alternate JournalChembiochem