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Screening of a protein that interacts with the matrix attachment region-binding protein from Dunaliella salina.
|Title||Screening of a protein that interacts with the matrix attachment region-binding protein from Dunaliella salina.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Yang R, Li Z, Lin Y, Yang B, Wang T|
|Journal||BioMed research international|
We isolated the matrix attachment region-binding protein (MBP) DMBP-1 from Dunaliella salina in our previous studies. MBPs are part of the cis-acting protein family cluster. The regulatory function possibly works through the interaction of the MBPs with each other. In the present study, DMBP-1 was used as the bait in screening the D. salina cDNA library for DMBP-1 interactors that could potentially mediate the DMBP-1-regulated functions. A novel MBP, namely, DMBP-2, was identified as a DMBP-1 binding partner. The cDNA of DMBP-1 was 823 bp long and contained a 573 bp open reading frame, which encoded a polypeptide of 191 amino acids. The interaction between DMBP-2 and DMBP-1 was further confirmed through glutathione S-transferase pull-down assays.
|Alternate Journal||Biomed Res Int|