Screening of a protein that interacts with the matrix attachment region-binding protein from Dunaliella salina.

TitleScreening of a protein that interacts with the matrix attachment region-binding protein from Dunaliella salina.
Publication TypeJournal Article
Year of Publication2013
AuthorsYang R, Li Z, Lin Y, Yang B, Wang T
JournalBioMed research international
Volume2013
Pagination862450
Date Published2013
Abstract

We isolated the matrix attachment region-binding protein (MBP) DMBP-1 from Dunaliella salina in our previous studies. MBPs are part of the cis-acting protein family cluster. The regulatory function possibly works through the interaction of the MBPs with each other. In the present study, DMBP-1 was used as the bait in screening the D. salina cDNA library for DMBP-1 interactors that could potentially mediate the DMBP-1-regulated functions. A novel MBP, namely, DMBP-2, was identified as a DMBP-1 binding partner. The cDNA of DMBP-1 was 823 bp long and contained a 573 bp open reading frame, which encoded a polypeptide of 191 amino acids. The interaction between DMBP-2 and DMBP-1 was further confirmed through glutathione S-transferase pull-down assays.

DOI10.1073/pnas.1313991110
Alternate JournalBiomed Res Int