Real-time dynamic optical imaging of ACC-M tumor cells killed by HSV-tk/ACV system.

TitleReal-time dynamic optical imaging of ACC-M tumor cells killed by HSV-tk/ACV system.
Publication TypeJournal Article
Year of Publication2013
AuthorsXiong T, Li Y, Li Z, Xie X, Lu L
JournalJournal of nanoscience and nanotechnology
Volume13
Issue1
Pagination123-8
Date Published2013 Jan
Abstract

HSV-tk/ACV induced and killed human adenoid cystic carcinoma cell (ACC-M) in vivo and in vitro, which were observed through optical imaging and green fluorescence protein (GFP) tagging technique. ACC-M was transfected with TK-GFP, and the single clone cell ACC-M-TK-GFP was selected by G418. With fluorescent stereomicroscope, whole-body fluorescent imaging system and fluorescent microscope, we could observe ACV treated ACC-M-TK-GFP cells in cell level and nude mice. The therapies of tumor were visualized clearly with optical imaging. This study proves that optical imaging is a very good approach for studying the effect of HSV-tk/ACV on the ACC-M tumor cells and decreasing the amount of vessel about tumors cell. Optical imaging will become a visual groundwork for monitoring tumor growth and evaluating in vivo curative effect of antitumor drugs.

DOI10.1111/tpj.12228
Alternate JournalJ Nanosci Nanotechnol