A quality control method for commercially available wild Jujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds.

TitleA quality control method for commercially available wild Jujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds.
Publication TypeJournal Article
Year of Publication2013
AuthorsZhang R, Chen J, Shi Q, Li Z, Peng Z, Zheng L, Wang X
JournalJournal of separation science
Date Published2013 Oct 26
Abstract

Flavonoids are the main active components of natural medicinal plants with many physiological functions. In this study, a HPLC fingerprinting method based on the distribution and relative amount of 11 bioactive flavonoids was established for the quality evaluation of commercially available wild Jujube leaf tea from China. Separation of the crude flavonoid extract was achieved on a column filled with C18 material with a high carbon content. The flavonoids in wild Jujube leaf tea were identified based on UV spectra and accurate mass measurements by TOF-MS. 21 batches of practical samples collected from different habitats were analyzed by using the developed HPLC method to construct the HPLC characteristic fingerprint of wild Jujube leaf tea. Then, combined with clustering and similarity analyses, the HPLC characteristic fingerprint was used for the authentication and quality evaluation of commercial wild Jujube leaf tea. Results indicated that the proposed HPLC characteristic fingerprint reflected the inherent characteristics of wild Jujube leaf tea collected from different regions. Authenticity identification and quality control of commercially available wild Jujube tea were achieved based on the HPLC characteristic fingerprint analysis. This new approach to bioactive component profiling provided a promising reference method for the quality evaluation of commercial wild flower and plant tea. This article is protected by copyright. All rights reserved.

DOI10.2337/dc13-1142
Alternate JournalJ Sep Sci