News & Updates
Search Research Content
Resource Finder at Kennedy Krieger Institute
A free resource that provides access to information and support for individuals and families living with developmental disabilities.
Preparation of functional magnetic nanoparticles mediated with PEG-4000 and application in Pseudomonas aeruginosa rapid detection.
|Title||Preparation of functional magnetic nanoparticles mediated with PEG-4000 and application in Pseudomonas aeruginosa rapid detection.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Tang Y, Li Z, He N, Zhang L, Ma C, Li X, Li C, Wang Z, Deng Y, He L|
|Journal||Journal of biomedical nanotechnology|
|Date Published||2013 Feb|
A rapid detection method of Pseudomonas aeruginosa based on magnetic separation and chemiluminescence was developed in this paper. Magnetic nanoparticles (MNPs) were prepared by solvothermal method with PEG-4000 as a surfactant, and then were modified. The prepared MNPs present a uniform morphology and good dispersion. The sizes of MNPs can be controlled by adjusting the dosage of FeCl3 x 6H2O. The obtained particles were characterized with Scanning electron microscope (SEM), Transmission electronic microscopy (TEM) and Fourier transform infrared (FTIR). The biotin-dUTP-labeled DNA fragments of gyrB gene were amplified by polymerase chain reaction (PCR), and Pseudomonas aeruginosa was successfully detected with detection limit as low as 7.5 fM of gyrB fragments.
|Alternate Journal||J Biomed Nanotechnol|