Preparation and application of an immunoaffinity column based on an antibody with strong affinity and packing material with good stability.

TitlePreparation and application of an immunoaffinity column based on an antibody with strong affinity and packing material with good stability.
Publication TypeJournal Article
Year of Publication2013
AuthorsZhang H, Wang M, Wang Y, Li Q, Zhou J, Huo F, Tang B
JournalFood additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment
Volume30
Issue5
Pagination853-60
Date Published2013
Abstract

This article describes the production of an anti-citrinin antibody that showed a high affinity constant (Ka) of 6.28 × 10⁹ and good tolerance to organic solvent and low pH, the synthesis of a Cu (II)-embedded polymer that showed strong binding with this antibody and the preparation of packing material for an immunoaffinity column (IAC) that show good stability. Most of the IACs reported either use harsh elution conditions and are used only once or use gentle elution conditions and are reused many times. Here, through the combined use of a strong-affinity antibody and packing material with good stability, high recoveries during clean-up and yet simultaneously good stability of the IAC were successfully achieved. Under optimised conditions of 80% methanol (pH 3), the IACs were used to clean-up the extracts of Monascus colour and red yeast rice samples, followed by HPLC detection. The recoveries of citrinin from spiked samples at levels of 50-200 μg kg⁻¹ were in the range of 84-97%.

DOI10.3969/j.issn.1672-7347.2013.06.013
Alternate JournalFood Addit Contam Part A Chem Anal Control Expo Risk Assess