Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein.

TitlePolymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein.
Publication TypeJournal Article
Year of Publication2013
AuthorsWang HL, Li ZX, Wang LJ, He H, Yang J, Chen L, Niu FB, Liu Y, Guo JZ, Liu XL
JournalResearch in veterinary science
Volume95
Issue2
Pagination508-14
Date Published2013 Oct
Abstract

Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T-35A, T-12G and G+102C were significantly associated (P<0.05) with somatic cell score (SCS), while G+102C and G+649C were significantly associated (P<0.05) with 305-day milk yield. Association analysis between combined haplotypes and SCS, milk production traits indicated that H3H3 was associated with the lower SCS (P<0.01), and H2H2 was associated with the lower 305-day milk yield (P<0.01). These findings demonstrated that polymorphisms in PGLYRP-1 gene associated with mastitis resistance and 305-day milk yield, and the H3H3 would provide a useful genetic marker of combined haplotypes for mastitis resistance selection and breeding in Chinese Holstein.

DOI10.1371/journal.pone.0067679
Alternate JournalRes. Vet. Sci.