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A novel density control device for the study of cancer cell autocrine effect.
|Title||A novel density control device for the study of cancer cell autocrine effect.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Yang W, Li Z, Zhang W, Luo C, Ouyang Q, Yang G, Wang Y|
|Date Published||2013 Aug|
In this paper, we propose a novel cell self-loading and patterning device for quantitatively study density effect on cell behaviors. Using this device, it is easy to gather different cell density colonies in different sizes of micro-chambers using one homogeneous cell solution. As a demonstration, we show that the cell number of self-patterning MCF-7 colony is in proportion to the size of liquid-absorbing cavity in the device, from single cell to tens of cells. This device can easily be used to compare the cancer cells' proliferation in different micro-environments, such as the same number of cells in micro-cavities with different sizes, or different numbers of cells in micro-cavities with the same size, or with different FBS concentrations. Our studies imply a plausible positive correlation between the local concentration of autocrine factors and tumor cell proliferation, which is also quantitative analyzed by a simple model.
|Alternate Journal||Biomed Microdevices|