NK cells are necessary for recovery of corneal CD11c+ dendritic cells after epithelial abrasion injury.

TitleNK cells are necessary for recovery of corneal CD11c+ dendritic cells after epithelial abrasion injury.
Publication TypeJournal Article
Year of Publication2013
AuthorsGao Y, Li ZJ, Hassan N, Mehta P, Burns AR, Tang X, Smith WC
JournalJournal of leukocyte biology
Volume94
Issue2
Pagination343-51
Date Published2013 Aug
Abstract

Mechanisms controlling CD11c(+) MHCII(+) DCs during corneal epithelial wound healing were investigated in a murine model of corneal abrasion. Selective depletion of NKp46(+) CD3- NK cells that normally migrate into the cornea after epithelial abrasion resulted in >85% reduction of the epithelial CD11c(+) MHCII(+) DCs, normally present during and after epithelial wound closure. Transfer (i.v.) of spleen NK cells into NK cell-depleted mice significantly restored levels of corneal epithelial DCs (P<0.01). Immigrated NK cells were predominately positive for IFN-γ, and topical corneal anti-IFN-γ reduced epithelial DCs by 79% (P<0.01). IFN-γ(-/-) mice had 69% fewer DCs than WT controls (P<0.01), and topical rIFN-γ applied to NK cell-depleted corneas increased epithelial DCs significantly (P<0.01). The contribution of ICAM-1, an adhesion molecule involved in leukocyte migration, expressed on healing corneal epithelium, was evaluated. ICAM-1(-/-) mice exhibited >70% reduction in epithelial DC recovery in the first 48 h after epithelial abrasion (P<0.01). These interventions reveal an early turnover of DCs in the epithelium after injury, and ICAM-1, NK cells, and IFN-γ are necessary for the immigration phase of this turnover.

DOI10.1002/jcb.24588
Alternate JournalJ. Leukoc. Biol.