Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor.

TitleNitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor.
Publication TypeJournal Article
Year of Publication2003
AuthorsMatsushita K, Morrell CN, Cambien B, Yang SX, Yamakuchi M, Bao C, Hara MR, Quick RA, Cao W, O'Rourke B, Lowenstein JM, Pevsner J, Wagner DD, Lowenstein CJ
JournalCell
Volume115
Issue2
Pagination139-50
Date Published2003 Oct 17
Abstract

Nitric oxide (NO) inhibits vascular inflammation, but the molecular basis for its anti-inflammatory properties is unknown. We show that NO inhibits exocytosis of Weibel-Palade bodies, endothelial granules that mediate vascular inflammation and thrombosis, by regulating the activity of N-ethylmaleimide-sensitive factor (NSF). NO inhibits NSF disassembly of soluble NSF attachment protein receptor (SNARE) complexes by nitrosylating critical cysteine residues of NSF. NO may regulate exocytosis in a variety of physiological processes, including vascular inflammation, neurotransmission, thrombosis, and cytotoxic T lymphocyte cell killing.

Alternate JournalCell