A new insect cell line from the pupal ovary of the Asian corn borer moth Ostrinia furnacalis.

TitleA new insect cell line from the pupal ovary of the Asian corn borer moth Ostrinia furnacalis.
Publication TypeJournal Article
Year of Publication2013
AuthorsZhang N, Qin Q, Gong H, Meng Q, Zhu W, Wang M, Zhang J, Zhou G, Li X, Zhang H
JournalIn vitro cellular & developmental biology. Animal
Date Published2013 Oct 26
Abstract

Ovaries were removed from Ostrinia furnacalis (Guenée) pupae and were placed in a flask containing TNM-FH medium with 10% inactivated fetal bovine serum. Cell migration occurred after about 1 wk of the initiation in June 2011. The migrated cells were distributed over most of the flask and were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a chemical carcinogen, after about 1 mo of initiation for 26 d. Cells were first subcultured successfully 12 d after the MNNG was removed, followed by subculturing for 30 passages. The established cell line, designated IOZCAS-Osfu-1, were analyzed by DNA fingerprinting-PCR (DAF-PCR) to confirm that it originated from O. furnacalis.

DOI10.1152/ajplung.00423.2012
Alternate JournalIn Vitro Cell. Dev. Biol. Anim.