MRI-detectable pH nanosensors incorporated into hydrogels for in vivo sensing of transplanted-cell viability.

TitleMRI-detectable pH nanosensors incorporated into hydrogels for in vivo sensing of transplanted-cell viability.
Publication TypeJournal Article
Year of Publication2013
AuthorsChan KWY, Liu G, Song X, Kim H, Yu T, Arifin DR, Gilad AA, Hanes J, Walczak P, van Zijl PCM, Bulte JWM, McMahon MT
JournalNature materials
Volume12
Issue3
Pagination268-75
Date Published2013 Mar
Abstract

Biocompatible nanomaterials and hydrogels have become an important tool for improving cell-based therapies by promoting cell survival and protecting cell transplants from immune rejection. Although their potential benefit has been widely evaluated, at present it is not possible to determine, in vivo, if and how long cells remain viable following their administration without the use of a reporter gene. Here, we report a pH-nanosensor-based magnetic resonance imaging (MRI) technique that can monitor cell death in vivo non-invasively. We demonstrate that specific MRI parameters that change on cell death of microencapsulated hepatocytes are associated with the measured bioluminescence imaging radiance. Moreover, the readout from this pH-sensitive nanosensor can be directly co-registered with high-resolution anatomical images. All of the components of these nanosensors are clinical grade and hence this approach should be a translatable and universal modification of hydrogels.

DOI10.1016/j.jviromet.2013.02.009
Alternate JournalNat Mater