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MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis.
|Title||MiR-29c inhibits glioma cell proliferation, migration, invasion and angiogenesis.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Fan Y-C, Mei P-J, Chen C, Miao F-A, Zhang H, Li Z-L|
|Journal||Journal of neuro-oncology|
|Date Published||2013 Nov|
Previous studies reported that miR-29c is significantly downregulated in several tumors. However, little is known about the effect and molecular mechanisms of action of miR-29c in human glioma. Using quantitative RT-PCR, we demonstrated that miR-29c was significantly downregulated in glioma cell lines and human primary glioma tissues, compared to normal human astrocytes and matched non-tumor associated tissues (P < 0.05, χ(2) test). Overexpression of miR-29c dramatically reduced the proliferation and caused cessation of cell cycle. The reduced cell proliferation is due to G1 phase arrest as cyclin D1 and cyclin E are diminished whereas p27 and p21 are upregulated. We further demonstrated that miR-29c overexpression suppressed the glioma cell migration and invasion abilities by targeting MMP-2. In addition, we also found that overexpression of miR-29c sharply inhibited angiogenesis, which correlated with down-regulation of VEGF. The data indicate that miR-29c may be a tumor suppressor involved in the progression of glioma.
|Alternate Journal||J. Neurooncol.|