miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression.

TitlemiR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression.
Publication TypeJournal Article
Year of Publication2013
AuthorsKang M, Li Y, Liu W, Wang R, Tang A, Hao H, Liu Z, Ou H
JournalInternational journal of molecular medicine
Volume32
Issue1
Pagination51-8
Date Published2013 Jul
Abstract

We report the emerging role of microRNA (miRNA) deregulation associated with activation of an oncogene SOX4 (a member of the SRY-related HMG-box) in esophageal carcinoma. Paired esophageal cancer and adjacent non-tumor tissues were obtained from 42 patients who underwent primary surgical resection for esophageal cancer. Experiments such as real-time PCR, western blot analysis, luciferase-reporter assay, cell proliferation and colony formation assays, in vitro migration and invasion assays, and a wound-healing assay were performed to determine the effects of miR-129-2. We found that SOX4 expression was elevated (P<0.005) in esophageal tumors (n=42) when compared with its expression in the controls (n=42). Compared with the normal esophageal tissues, the expression of miR-129-2 was downregulated in 27 of 31 primary esophageal tumors, while the expression of SOX4 was upregulated (P<0.001). Restoration of miR-129-2 by transfection with an miRNA expression plasmid led to a decrease in SOX4 expression, which was accompanied by reduced migration and proliferation of the cancer cells. These results suggest that aberrant expression of SOX4 is associated with repression of miR-129-2, and restoration of miR-129-2 suppresses the migration and proliferation of esophageal cancer cells. Our results demonstrated that the deregulation of miR-129-2 leads to aberrant SOX4 expression, presenting a new paradigm in which the restoration of miRNA suppresses its oncogenic target in esophageal cancer.

DOI10.5603/CJ.a2013.0057
Alternate JournalInt. J. Mol. Med.