Metagenomic approaches for exploiting uncultivated bacteria as a resource for novel biosynthetic enzymology.

TitleMetagenomic approaches for exploiting uncultivated bacteria as a resource for novel biosynthetic enzymology.
Publication TypeJournal Article
Year of Publication2013
AuthorsWilson MC, Piel J
JournalChemistry & biology
Volume20
Issue5
Pagination636-47
Date Published2013 May 23
Abstract

Most biologically active microbial natural products are known from strains that can be isolated and cultivated in the laboratory. However, the genomics era has revealed that cultured bacteria represent a mere fraction of total estimated bacterial biodiversity. With the development of community genomics, termed metagenomics, the uncultivated majority became accessible for functional analysis. Through metagenomic studies, novel biocatalysts and biosynthetic pathways are being discovered at a pace previously not possible using traditional molecular biology techniques. Additionally, the study of uncultivated bacteria has provided valuable insights into previously overlooked biocatalysts from cultured strains. This perspective highlights recent discoveries from metagenomics of uncultivated bacteria and discusses the impact of those findings on the field of natural products.

DOI10.1002/anie.201303507
Alternate JournalChem. Biol.