Mass spectrometry-based detection of protein acetylation.

TitleMass spectrometry-based detection of protein acetylation.
Publication TypeJournal Article
Year of Publication2013
AuthorsLi Y, Silva JC, Skinner ME, Lombard DB
JournalMethods in molecular biology (Clifton, N.J.)
Date Published2013

Improved sample preparation techniques and increasingly sensitive mass spectrometry (MS) analysis have revolutionized the study of protein post-translational modifications (PTMs) (Rush et al., Nat Biotechnol 23:94-101, 2005). Here, we describe a general approach for immunopurification and MS-based identification of acetylated proteins in biological samples. This approach is useful to characterize changes in the acetylome in response to biological interventions (Schwer et al., Aging Cell 8:604-606, 2009).

Alternate JournalMethods Mol. Biol.