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Mass spectrometry-based detection of protein acetylation.
|Title||Mass spectrometry-based detection of protein acetylation.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Li Y, Silva JC, Skinner ME, Lombard DB|
|Journal||Methods in molecular biology (Clifton, N.J.)|
Improved sample preparation techniques and increasingly sensitive mass spectrometry (MS) analysis have revolutionized the study of protein post-translational modifications (PTMs) (Rush et al., Nat Biotechnol 23:94-101, 2005). Here, we describe a general approach for immunopurification and MS-based identification of acetylated proteins in biological samples. This approach is useful to characterize changes in the acetylome in response to biological interventions (Schwer et al., Aging Cell 8:604-606, 2009).
|Alternate Journal||Methods Mol. Biol.|