Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay.

TitleIsolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay.
Publication TypeJournal Article
Year of Publication2013
AuthorsWang F, Scoville D, He XC, Mahe MM, Box A, Perry JM, Smith NR, Lei N, Davies PS, Fuller MK, Haug JS, McClain M, Gracz AD, Ding S, Stelzner M, Dunn JCY, Magness ST, Wong MH, Martin MG, Helmrath M, Li L
JournalGastroenterology
Volume145
Issue2
Pagination383-95.e1-21
Date Published2013 Aug
Abstract

Identification of intestinal stem cells (ISCs) has relied heavily on the use of transgenic reporters in mice, but this approach is limited by mosaic expression patterns and difficult to directly apply to human tissues. We sought to identify reliable surface markers of ISCs and establish a robust functional assay to characterize ISCs from mouse and human tissues.

DOI10.1038/nm.3162
Alternate JournalGastroenterology