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Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay.
|Title||Isolation and characterization of intestinal stem cells based on surface marker combinations and colony-formation assay.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Wang F, Scoville D, He XC, Mahe MM, Box A, Perry JM, Smith NR, Lei N, Davies PS, Fuller MK, Haug JS, McClain M, Gracz AD, Ding S, Stelzner M, Dunn JCY, Magness ST, Wong MH, Martin MG, Helmrath M, Li L|
|Date Published||2013 Aug|
Identification of intestinal stem cells (ISCs) has relied heavily on the use of transgenic reporters in mice, but this approach is limited by mosaic expression patterns and difficult to directly apply to human tissues. We sought to identify reliable surface markers of ISCs and establish a robust functional assay to characterize ISCs from mouse and human tissues.