Imaging protein three-dimensional nanocrystals with cryo-EM.

TitleImaging protein three-dimensional nanocrystals with cryo-EM.
Publication TypeJournal Article
Year of Publication2013
AuthorsNederlof I, Li YW, van Heel M, Abrahams JP
JournalActa crystallographica. Section D, Biological crystallography
IssuePt 5
Date Published2013 May

Flash-cooled three-dimensional crystals of the small protein lysozyme with a thickness of the order of 100 nm were imaged by 300 kV cryo-EM on a Falcon direct electron detector. The images were taken close to focus and to the eye appeared devoid of contrast. Fourier transforms of the images revealed the reciprocal lattice up to 3 Å resolution in favourable cases and up to 4 Å resolution for about half the crystals. The reciprocal-lattice spots showed structure, indicating that the ordering of the crystals was not uniform. Data processing revealed details at higher than 2 Å resolution and indicated the presence of multiple mosaic blocks within the crystal which could be separately processed. The prospects for full three-dimensional structure determination by electron imaging of protein three-dimensional nanocrystals are discussed.

Alternate JournalActa Crystallogr. D Biol. Crystallogr.