Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis.

TitleIdentification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis.
Publication TypeJournal Article
Year of Publication2013
AuthorsFeng L, Yu Q, Li X, Ning X, Wang J, Zou J, Zhang L, Wang S, Hu J, Hu X, Bao Z
JournalPloS one
Volume8
Issue9
Paginatione75609
Date Published2013
Abstract

Bivalves comprise around 30,000 extant species and have received much attention for their importance in ecosystems, aquaculture and evolutionary studies. Despite the increasing application of real-time quantitative reverse transcription PCR (qRT-PCR) in gene expression studies on bivalve species, little research has been conducted on reference gene selection which is critical for reliable and accurate qRT-PCR analysis. For scallops, systematic evaluation of reference genes that can be used among tissues or embryo/larva stages is lacking, and β-actin (ACT) is most frequently used as qRT-PCR reference gene without validation.

DOI10.1155/2013/361906
Alternate JournalPLoS ONE