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Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis.
|Title||Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Feng L, Yu Q, Li X, Ning X, Wang J, Zou J, Zhang L, Wang S, Hu J, Hu X, Bao Z|
Bivalves comprise around 30,000 extant species and have received much attention for their importance in ecosystems, aquaculture and evolutionary studies. Despite the increasing application of real-time quantitative reverse transcription PCR (qRT-PCR) in gene expression studies on bivalve species, little research has been conducted on reference gene selection which is critical for reliable and accurate qRT-PCR analysis. For scallops, systematic evaluation of reference genes that can be used among tissues or embryo/larva stages is lacking, and β-actin (ACT) is most frequently used as qRT-PCR reference gene without validation.
|Alternate Journal||PLoS ONE|