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Identification of differential expressed proteins and characterization their mRNA expression in thermally stressed Apostichopus japonicus.
|Title||Identification of differential expressed proteins and characterization their mRNA expression in thermally stressed Apostichopus japonicus.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Zhang P, Lu Y, Li C, Su X, Wang Z, Jin C, Li Y, Li T|
|Journal||Comparative biochemistry and physiology. Part D, Genomics & proteomics|
|Date Published||2013 Sep|
In this study, we present a comparative proteomic analysis of the global protein expression changes in sea cucumber after 7 days exposure at 25°C. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 27 protein spots with significant differences in abundance were identified and characterized. The identified proteins belonged primarily to the following four functional categories: cytoskeletal, material and energy metabolism, calcium homeostasis and extracellular matrix. The mRNA expression levels of 7 differentially expressed proteins were further assessed by qRT-PCR. The expression levels of 6 genes, including collagen, ATP synthase, major yolk protein, ferritin, nectin and protein disulfide isomerase showed significant differences under thermal stress, and among them, only two genes-ATP synthase and major yolk protein-showed consistent levels of protein and mRNA expression. Our results offer insight into the complex changes in protein turnover during higher temperature exposure in sea cucumber.
|Alternate Journal||Comp. Biochem. Physiol. Part D Genomics Proteomics|