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Identification and characterization of the first Escherichia coli strain carrying NDM-1 gene in China.
|Title||Identification and characterization of the first Escherichia coli strain carrying NDM-1 gene in China.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Liu Z, Li W, Wang J, Pan J, Sun S, Yu Y, Zhao B, Ma Y, Zhang T, Qi J, Liu G, Lu F|
New Delhi metallo-β-lactamase-1 (NDM-1), an acquired class B carbapenemase, is a significant clinical threat due to its extended hydrolysis of β-lactams including carbapenems. In this study, we identified the first confirmed clinical isolate of Escherichia coli BJ01 harboring bla(NDM-1) in China. The isolate is highly resistant to all tested antimicrobials except polymyxin. bla(NDM-1), bla(CTX-M-57), and bla TEM-1 were identified in the isolate. bla(NDM-1) was transferable to E. coli EC600 and DH5α in both plasmid conjugation experiments and plasmid transformation tests. BJ01 was identified as a new sequence type, ST224, by multilocus sequence typing. Analysis of genetic environment shows complex transposon-like structures surrounding the bla NDM-1 gene. Genetic analysis revealed that the region flanking bla(NDM-1) was very similar to previously identified NDM-positive Acinetobacter spp. isolated in China. The findings of this study raise attention to the emergence and spread of NDM-1-carrying Enterobacteriaceae in China.
|Alternate Journal||PLoS ONE|