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Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus.
|Title||Identification and assessment of differentially expressed genes involved in growth regulation in Apostichopus japonicus.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Zhu L, Li CH, Su XR, Guo CY, Wang Z, Jin CH, Li Y, Li TW|
|Journal||Genetics and molecular research : GMR|
Rapid and efficient growth is a major consideration and challenge for global mariculture. The differential growth rate of the sea cucumber, Apostichopus japonicus, has significantly hampered the total production of the industry. In the present study, forward and reverse suppression subtractive hybridization libraries were constructed and sequenced from a fast-growth group and a slow-growth group of the sea cucumber. A total of 142 differentially expressed sequence tags (ESTs) with insertions longer than 150 bp were identified and further analyzed. Fifty-seven of these ESTs (approximately 40%) were functionally annotated for cell structure, energy metabolism, immunity response, and growth factor categories. Six candidate genes, arginine kinase, cytochrome c oxidase subunit I, HSP70, β-actin, ferritin, and the ADP-ribosylation factor, were further validated by quantitative PCR. Significant differences were found between the fast- and slow-growth groups (P < 0.05) for the expression levels of arginine kinase, cytochrome c oxidase, HSP70, the ADP-ribosylation factor, and β-actin. However, no significant difference was observed for ferritin. Our results provide promising candidate gene markers for practical size screening, and also further promote marker-assisted selective breeding of this species.
|Alternate Journal||Genet. Mol. Res.|