Icariin delivery porous PHBV scaffolds for promoting osteoblast expansion in vitro.

TitleIcariin delivery porous PHBV scaffolds for promoting osteoblast expansion in vitro.
Publication TypeJournal Article
Year of Publication2013
AuthorsXia L, Li Y, Zhou Z, Dai Y, Liu H, Liu H
JournalMaterials science & engineering. C, Materials for biological applications
Volume33
Issue6
Pagination3545-52
Date Published2013 Aug 1
Abstract

How cells could proliferate quickly and maintain their biological activity by using appropriate scaffolds remains a big challenge for tissue engineering. By integrating icariin, a bioactive ingredient of traditional Chinese medicine (TCM) Epimedii herba, with PHBV scaffolds, novel icariin delivery porous PHBV scaffolds (IDPPSs) were fabricated with a combination of the solvent casting and salt leaching techniques. IDPPSs displayed a high porosity, 88.80%, and appropriate mechanical properties which were required for 3-D cell culture. IDPPSs significantly promoted the proliferation of human osteoblast-like MG-63 cells and the pre-osteoblast MC3T3-E1 cells, while IDPPSs containing 0.1% icariin (wt.%) showed the highest effect compared with other samples. Although IDPPSs continuously released icariin to the solution in 28 days, cells attached to IDPPSs received an enhanced growth stimulation compared with which were not physically in contact with IDPPSs. Up-regulated transcription of growth factor genes and extracellular matrix genes, including BMP2, BMP6, BMP7 and BGN, was observed in IDPPS-cultured MG-63 cells, illustrating that enhanced cellular proliferation results from alteration of gene transcription. These results implied the potential commercial use of IDPPSs in tissue engineering.

DOI10.1039/c3cc42962h
Alternate JournalMater Sci Eng C Mater Biol Appl