Hematotoxicological analysis of surface-modified and -unmodified chitosan nanoparticles.

TitleHematotoxicological analysis of surface-modified and -unmodified chitosan nanoparticles.
Publication TypeJournal Article
Year of Publication2013
AuthorsNadesh R, Narayanan D, P R S, Vadakumpully S, Mony U, Koyakkutty M, Nair SV, Menon D
JournalJournal of biomedical materials research. Part A
Volume101
Issue10
Pagination2957-66
Date Published2013 Oct
Abstract

The increasing interest in using chitosan nanoparticles for controlled drug delivery is hampered by its blood incompatibility, especially for intravenous applications. This study investigated the effects of processing solvents (acetic acid/lactic acid), dispersing media (acidic medium/saline), and surface modifiers (polyethylene glycol, polyvinyl alcohol, and ethylenediaminetetraacetatic acid) on the hemocompatibility of chitosan. Blood compatibility of chitosan nanoparticles prepared by ionotropic gelation with altered surface chemistry was evaluated by assessing their hemolytic activity, platelet aggregation, coagulation, and cytokine induction. It was observed that nanoparticles prepared in lactic acid and dispersed in saline did not show hemolysis, platelet aggregation, or coagulation, whereas nanoparticles prepared in acetic acid showed strong hemolysis. Surface modifiers were not observed to significantly affect blood compatibility, with the exception of EDTA, which delayed blood clotting times. Thus, chitosan nanoparticles prepared in lactic acid and dispersed in saline may be an ideal nanocarrier for parenteral applications.

DOI10.1371/journal.pone.0061053
Alternate JournalJ Biomed Mater Res A