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Glucose responsive insulin production from human embryonic germ (EG) cell derivatives.
|Title||Glucose responsive insulin production from human embryonic germ (EG) cell derivatives.|
|Publication Type||Journal Article|
|Year of Publication||2007|
|Authors||Clark GO, Yochem RL, Axelman J, Sheets TP, Kaczorowski DJ, Shamblott MJ|
|Journal||Biochemical and biophysical research communications|
|Date Published||2007 May 11|
Type 1 diabetes mellitus subjects millions to a daily burden of disease management, life threatening hypoglycemia and long-term complications such as retinopathy, nephropathy, heart disease, and stroke. Cell transplantation therapies providing a glucose-regulated supply of insulin have been implemented clinically, but are limited by safety, efficacy and supply considerations. Stem cells promise a plentiful and flexible source of cells for transplantation therapies. Here, we show that cells derived from human embryonic germ (EG) cells express markers of definitive endoderm, pancreatic and beta-cell development, glucose sensing, and production of mature insulin. These cells integrate functions necessary for glucose responsive regulation of preproinsulin mRNA and expression of insulin C-peptide in vitro. Following transplantation into mice, cells become insulin and C-peptide immunoreactive and produce plasma C-peptide in response to glucose. These findings suggest that EG cell derivatives may eventually serve as a source of insulin producing cells for the treatment of diabetes.
|Alternate Journal||Biochem. Biophys. Res. Commun.|