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FLUORESCENCE KINETICS OF COMPLEX FORMATION BETWEEN HUMAN SERUM ALBUMIN AND ZINC-PHTHALOCYANINE TETRASULFONIC ACID.
|Title||FLUORESCENCE KINETICS OF COMPLEX FORMATION BETWEEN HUMAN SERUM ALBUMIN AND ZINC-PHTHALOCYANINE TETRASULFONIC ACID.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Jones CL, Fields A, Thomas L, Patel S, Biggins S|
|Journal||Journal of undergraduate chemistry research|
|Date Published||2011 Winter|
Stopped-flow fluorescence was employed to measure the dissociation constant, activation energy, and frequency factor for zinc phthalocyanine tetrasulfonic acid binding to human serum albumin. A pseudo 1(st) order "Isolation Method" was used to measure temperature dependent rate constants at pH 7.00. Binding was followed by a relatively large emission signal that indicated the formation of a protein-ligand complex. The dissociation constant, activation energy and frequency factor were 3.3 (±0.3)×10(-5) (M), 4.5 (±0.2)×10(4) J/mol, and 1.1 (±0.1)×10(8) min(-1) respectively.
|Alternate Journal||J Undergrad Chem Res|