Establishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells.

TitleEstablishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells.
Publication TypeJournal Article
Year of Publication2013
AuthorsXu J, Yoshimura K, Mon H, Li Z, Zhu L, Iiyama K, Kusakabe T, Lee JM
JournalMolecular biotechnology
Date Published2013 Aug 24
Abstract

The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

DOI10.1155/2013/756012
Alternate JournalMol. Biotechnol.