An engineered HIV-1 gp41 trimeric coiled coil with increased stability and anti-HIV-1 activity: implication for developing anti-HIV microbicides.

TitleAn engineered HIV-1 gp41 trimeric coiled coil with increased stability and anti-HIV-1 activity: implication for developing anti-HIV microbicides.
Publication TypeJournal Article
Year of Publication2013
AuthorsTong P, Lu Z, Chen X, Wang Q, Yu F, Zou P, Yu X, Li Y, Lu L, Chen Y-H, Jiang S
JournalThe Journal of antimicrobial chemotherapy
Volume68
Issue11
Pagination2533-44
Date Published2013 Nov
Abstract

We previously constructed a trimeric coiled coil, N28Fd, based on the N-heptad repeat (NHR) sequence of HIV-1 gp41, as a promising HIV-1 entry inhibitor. Here, we attempted to engineer a stabilized trimeric coiled coil, ccN28Fd, by adding interchain disulphide bonds at the N terminus of N28Fd to improve its biophysical properties and anti-HIV-1 efficacy.

DOI10.1093/jac/dkt230
Alternate JournalJ. Antimicrob. Chemother.