Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture.

TitleEmbryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture.
Publication TypeJournal Article
Year of Publication1994
AuthorsCao MQ, Li Y, Liu F, Doré C
JournalPlant cell reports
Volume13
Issue8
Pagination447-50
Date Published1994 May
Abstract

Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33°C one day heat treatment was generally necessary to induce embryogenesis. Analysis of ploidy level through flow cytometry for two cultivars indicated that haploids were present.

DOI10.1155/2013/952350
Alternate JournalPlant Cell Rep.