The effect of amino density on the attachment, migration, and differentiation of rat neural stem cells In Vitro.

TitleThe effect of amino density on the attachment, migration, and differentiation of rat neural stem cells In Vitro.
Publication TypeJournal Article
Year of Publication2013
AuthorsLi H-L, Zhang H, Huang H, Liu Z-Q, Li Y-B, Yu H, An Y-H
JournalMolecules and cells
Volume35
Issue5
Pagination436-43
Date Published2013 May
Abstract

Artificial extracellular matrices play important roles in the regulation of stem cell behavior. To generate materials for tissue engineering, active functional groups, such as amino, carboxyl, and hydroxyl, are often introduced to change the properties of the biomaterial surface. In this study, we chemically modified coverslips to create surfaces with different amino densities and investigated the adhesion, migration, and differentiation of neural stem cells (NSCs) under serum-free culture conditions. We observed that a higher amino density significantly promoted NSCs attachment, enhanced neuronal differentiation and promoted excitatory synapse formation in vitro. These results indicate that the amino density significantly affected the biological behavior of NSCs. Thus, the density and impact of functional groups in extracellular matrices should be considered in the research and development of materials for tissue engineering.

DOI10.1371/journal.pone.0081430
Alternate JournalMol. Cells