De novo assembly of the sea cucumber Apostichopus japonicus hemocytes transcriptome to identify miRNA targets associated with skin ulceration syndrome.

TitleDe novo assembly of the sea cucumber Apostichopus japonicus hemocytes transcriptome to identify miRNA targets associated with skin ulceration syndrome.
Publication TypeJournal Article
Year of Publication2013
AuthorsZhang P, Li C, Zhu L, Su X, Li Y, Jin C, Li T
JournalPloS one
Volume8
Issue9
Paginatione73506
Date Published2013
Abstract

De novo transcriptome sequencing is a robust method of predicting miRNA target genes, especially samples without reference genomes. Differentially expressed miRNAs have been previously identified in hemocytes collected from healthy skin and from skin affected by skin ulceration syndrome (SUS) in Apostichopusjaponicus. Target identification for these differentially expressed miRNAs is a major challenge for this non-model organism.

DOI10.1007/s12011-013-9822-5
Alternate JournalPLoS ONE