Cotton PRP5 gene encoding a proline-rich protein is involved in fiber development.

TitleCotton PRP5 gene encoding a proline-rich protein is involved in fiber development.
Publication TypeJournal Article
Year of Publication2013
AuthorsXu W-L, Zhang D-J, Wu Y-F, Qin L-X, Huang G-Q, Li J, Li L, Li X-B
JournalPlant molecular biology
Volume82
Issue4-5
Pagination353-65
Date Published2013 Jul
Abstract

Proline-rich proteins contribute to cell wall structure of specific cell types and are involved in plant growth and development. In this study, a fiber-specific gene, GhPRP5, encoding a proline-rich protein was functionally characterized in cotton. GhPRP5 promoter directed GUS expression only in trichomes of both transgenic Arabidopsis and tobacco plants. The transgenic Arabidopsis plants with overexpressing GhPRP5 displayed reduced cell growth, resulting in smaller cell size and consequently plant dwarfs, in comparison with wild type plants. In contrast, knock-down of GhPRP5 expression by RNA interference in cotton enhanced fiber development. The fiber length of transgenic cotton plants was longer than that of wild type. In addition, some genes involved in fiber elongation and wall biosynthesis of cotton were up-regulated or down-regulated in the transgenic cotton plants owing to suppression of GhPRP5. Collectively, these data suggested that GhPRP5 protein as a negative regulator participates in modulating fiber development of cotton.

DOI10.1371/journal.pone.0062610
Alternate JournalPlant Mol. Biol.