Cnuella takakiae gen. nov., sp. nov., a novel member of phylum Bacteroidetes isolated from Takakia lepidozioides.

TitleCnuella takakiae gen. nov., sp. nov., a novel member of phylum Bacteroidetes isolated from Takakia lepidozioides.
Publication TypeJournal Article
Year of Publication2013
AuthorsZhao R, Chen XY, Li XD, Tian Y, Kong BH, Chen ZL, Li Y
JournalInternational journal of systematic and evolutionary microbiology
Date Published2013 Nov 6
Abstract

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1T, was isolated from Takakia lepidozioides collected from Gawalong glacier in Tibet, China and characterized by using polyphasic taxonomic approach. The predominant fatty acids of strain RG1-1T were iso-C15:0 (19.8 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c, 17.0 %), C16:0 (9.9 %) and iso-C17:0 3-OH (9.4 %); its major polar lipids were phosphatidylethanolamine, four unidentified aminolipids, one unidentified phospholipid, one unidentified aminoglycolipid, one unidentified glycolipid, and three unidentified lipids. Strain RG1-1T contained MK-7 as the dominant menaquinone, and the G+C content of its genomic DNA was 49.1 mol%. Strain RG1-1T exhibited the highest 16S rRNA gene sequence similarity (91.8 %) with Flavisolibacter ginsengiterrae Gsoil 492T and Flavisolibacter ginsengisoil Gsoil 643T. Phylogenetic analysis showed that strain RG1-1T was a member of the family Chitinophagaceae, phylum Bacteroidetes. Based on the 16S rRNA gene sequence analysis, phenotypic and chemotaxonomic data, strain RG1-1T is considered to represent a novel species of a novel genus, for which the name Cnuella takakiae gen. nov., sp. nov. is proposed. The type strain is RG1-1T (= CGMCC 1.12492T = DSM 26897T).

DOI10.1155/2013/952350
Alternate JournalInt. J. Syst. Evol. Microbiol.