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Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1.
|Title||Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Zhang D, Zhao Y, He Y, Wang Y, Zhao Y, Zheng Y, Wei X, Zhang L, Li Y, Jin T, Wu L, Wang H, Davison PA, Xu J, Huang WE|
|Journal||ACS synthetic biology|
|Date Published||2012 Jul 20|
Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.
|Alternate Journal||ACS Synth Biol|