Cellodextrin transporters play important roles in cellulase induction in the cellulolytic fungus Penicillium oxalicum.

TitleCellodextrin transporters play important roles in cellulase induction in the cellulolytic fungus Penicillium oxalicum.
Publication TypeJournal Article
Year of Publication2013
AuthorsLi J, Liu G, Chen M, Li Z, Qin Y, Qu Y
JournalApplied microbiology and biotechnology
Volume97
Issue24
Pagination10479-88
Date Published2013 Dec
Abstract

Cellodextrin transporters (cellodextrin permeases) have been identified in fungi in recent years. However, the functions of these transporters in cellulose utilization and cellulase expression have not been well studied. In this study, three cellodextrin transporters, namely, CdtC, CdtD, and CdtG, in the cellulolytic fungus Penicillium oxalicum (formally was classified as P. decumbens) were identified, and their functions were analyzed. The deletion of a single cellodextrin transporter gene slightly decreased cellobiose consumption, but no observable effect on cellulase expression was observed, which was attributed to the overlapping activity of isozymes. Further simultaneous deletion of cdtC and cdtD resulted in significantly decreased cellobiose consumption and poor growth on cellulose. The extracellular activity and transcription level of cellulases in the mutant without cdtC and cdtD were significantly lower than those in the wild-type strain when grown on cellulose. This result provides direct evidence of the crucial function of cellodextrin transporters in the induction of cellulase expression by insoluble cellulose.

DOI10.1039/c3an01381b
Alternate JournalAppl. Microbiol. Biotechnol.