Cell senescence culturing methods.

TitleCell senescence culturing methods.
Publication TypeJournal Article
Year of Publication2013
AuthorsChen H, Li Y, Tollefsbol TO
JournalMethods in molecular biology (Clifton, N.J.)
Volume1048
Pagination1-10
Date Published2013
Abstract

Development of therapeutic approaches that slow or ablate the adverse physiological and pathological changes associated with aging has been considered as an important goal for gerontological research. As cellular senescence is characterized as the basis for aging in organisms, culturing and subculturing of normal human diploid fibroblasts to mimic the in vivo aging processes have been developed as major methods to investigate molecular events involved in aging. It has been established that normal human diploid fibroblasts can proliferate in culture for only finite periods of time. There are many ways to study aging in vitro. In this chapter, we will discuss some of the basic laboratory procedures for cell senescence culturing methods.

DOI10.1002/hep.26667
Alternate JournalMethods Mol. Biol.