Bidirectional Immunomodulatory Activities of Polysaccharides Purified From Pleurotus nebrodensis.

TitleBidirectional Immunomodulatory Activities of Polysaccharides Purified From Pleurotus nebrodensis.
Publication TypeJournal Article
Year of Publication2013
AuthorsWang C, Cui H, Wang Y, Wang Z, Li Z, Chen M, Li F
JournalInflammation
Date Published2013 Aug 21
Abstract

A novel Pleurotus nebrodensis polysaccharide (PN50G) was purified, characterized, and cultured with RAW264.7 macrophages to evaluate its bidirectional immunostimulating characteristics. PN50G was purified by using Sepharose 4B gel filtration; the molecular weight of PN50G was distributed at 2,000 kDa. The total protein and carbohydrate constituent ratios in PN50G were 2.6 ± 0.9 % and 92.4 ± 6.1 % (w/w), respectively, which suggests that PN50G may be a major proteo-polysaccharide component. The phagocytosis of macrophages was improved significantly, and remarkable changes were observed in the morphology of PN50G-treated cells. Compared with the control group, the productions of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), and inducible nitric oxide synthase (iNOS) in the macrophages as well as the messenger RNA expressions were strongly induced by PN50G. Pro-/anti-inflammatory (IL-6/IL-10, TNF-α/IL-10, NO/IL-10) cytokine secretion ratios by lipopolysaccharide-stimulated RAW264.7 macrophages were significantly decreased by PN50G treatments in a dose-dependent manner under an excessive immune experimental model. This study suggests that purified PN50G can improve immunity and suppress immune overactivity in LPS-induced macrophages in a preventive manner to coordinate innate immunity and inflammatory responses.

DOI10.1039/c3cc44336a
Alternate JournalInflammation