Artificial reporter gene providing MRI contrast based on proton exchange.

TitleArtificial reporter gene providing MRI contrast based on proton exchange.
Publication TypeJournal Article
Year of Publication2007
AuthorsGilad AA, McMahon MT, Walczak P, Winnard PT, Raman V, van Laarhoven HWM, Skoglund CM, Bulte JWM, van Zijl PCM
JournalNature biotechnology
Volume25
Issue2
Pagination217-9
Date Published2007 Feb
Abstract

Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.

DOI10.1021/jo800879e
Alternate JournalNat. Biotechnol.