Guanshu Liu, Ph.D.

Guanshu Liu
Guanshu Liu
Research Scientist, F.M. Kirby Center

Kennedy Krieger Institute
707 N. Broadway
Baltimore, MD 21205
Phone: (443) 287-8306
Email: guanshu@mri.jhu.edu or liug@kennedykrieger.org

Guanshu Liu is an Assistant Professor in the F.M. Kirby Center at Kennedy Krieger Institute. He holds a joint appointment as Assistant Professor in the Russell H. Morgan Department of Radiology at Johns Hopkins University.


Biographical Sketch:

Guanshu Liu, Ph.D., is currently a tenure track Assistant Professor in the F.M. Kirby Center at the Kennedy Krieger Institute and the Russell H. Morgan Department of Radiology and Radiological Science at Johns Hopkins Medical School. Dr. Guanshu Liu earned his doctoral degree in biomedical engineering at Case Western Reserve University. Then he joined Dr. Peter van Zijl's imaging center at Johns Hopkins University in 2008 as a Research Associate.


Research Summary: 

The primary goal of my research is to develop novel Magnetic Resonance (MR) molecular imaging techniques to facilitate the development of modern medicine. As a relatively young but revolutionary discipline, MR molecular imaging aims at expanding traditional MRI from a macroscopic anatomical scale to a cellular and subcellular scale on which specific molecular markers can be specifically visualized, characterized and quantified in correspondence to a disease stage or a treatment response. As such, MR molecular imaging plays a key role in translating those discoveries in basic biomedical researches to clinical practices. My current researches mainly focus on the development and applications of a novel MRI technique, so called Chemical Exchange Saturation Transfer (CEST). We are currently developing a variety of nanoparticle-based CEST imaging probes with low toxicity, high sensitivity and high specificity, and exploring the biomedical applications of these new imaging probes. In addition, we are also interested in exploring the biomedical uses of endogenous CEST contrast. For example, one of ongoing projects in my lab focuses on exploiting the endogenous CEST MRI signal of bacterial organisms for detecting and monitoring bacterial infection in rodent models.

LINK: SciVal Experts Research Profile for Guanshu Liu


Research Publications

Liu G, Bettegowda C, Qiao Y, Staedtke V, Chan KW, Bai R, Li Y, Riggins GJ, Kinzler KW, Bulte JW, McMahon MT, Gilad AA, Vogelstein B, Zhou S, van Zijl PC. (2013). Noninvasive imaging of infection after treatment with tumor-homing bacteria using Chemical Exchange Saturation Transfer (CEST) MRI. Magnetic Resonance in Medicine, 70(6), 1690-1698.

Bar-Shir A, Gilad AA, Chan KW, Liu G, van Zijl PC, Bulte JW, McMahon MT. (2013). Metal ion sensing using ion chemical exchange saturation transfer 19F magnetic resonance imaging. Journal of the American Chemical Society, 135(33), 12164-12167.

Yang X, Song X, Li Y, Liu G, Ray Banerjee S, Pomper MG, McMahon MT. (2013). Salicylic acid and analogues as diaCEST MRI contrast agents with highly shifted exchangeable proton frequencies. Angewandte Chemie (International Education in English), 52(31), 816-819.

Chan KW, Liu G, Song X, Kim H, Yu T, Arifin DR, Gilad AA, Hanes J, Walczak P, van Zijl PC, Bulte JW, McMahon MT. (2013). MRI-detectable pH nanosensors incorporated into hydrogels for in vivo sensing of transplanted-cell viability. Nature Materials, 12(3), 268-275.

Liu G, Song X, Chan KW, McMahon MT. (2013). Nuts and bolts of chemical exchange saturation transfer MRI. NMR in Biomedicine, 26(7), 810-828.

Bar-Shir A, Liu G, Liang Y, Yadav NN, McMahon MT, Walczak P, Nimmagadda S, Pomper MG, Tallman KA, Greenberg MM, van Zijl PC, Bulte JW, Gilad AA. (2013). Transforming thymidine into a magnetic resonance imaging probe for monitoring gene expression. Journal of the American Chemical Society, 135(4), 1617-1624.

Liu G, Chan KW, Song X, Zhang J, Gilad AA, Bulte JW, van Zijl PC, McMahon MT. (2013). NOrmalized MAgnetization Ratio (NOMAR) filtering for creation of tissue selective contrast mapsMagnetic Resonance in Medicine, 69(2), 516-523.

Chan KW, McMahon MT, Kato Y, Liu G, Bulte JW, Bhujwalla ZM, Artemov D, van Zijl PC. (2012). Natural D-glucose as a biodegradable MRI contrast agent for detecting cancer. Magnetic Resonance in Medicine, 68(6), 1764-1773.

Airan RD, Bar-Shir A, Liu G, Pelled G, McMahon MT, van Zijl PC, Bulte JW, Gilad AA. (2012). MRI biosensor for protein kinase A encoded by a single synthetic gene. Magnetic Resonance in Medicine, 68(6), 1919-1923. 

Liu G, Li Y, Sheth VR, Pagel MD. (2012). Imaging in vivo extracellular pH with a single paramagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent. Molecular Imgaging, 11(1), 47-57.

Liu G, Moake M, Har-el YE, Long CM, Chan KW, Cardona A, Jamil M, Walczak P, Gilad AA, Sgouros G, van Zijl PC, Bulte JW, McMahon MT. (2012). In vivo multicolor molecular MR imaging using diamagnetic chemical exchange saturation transfer liposomes. Magnetic Resonance in Medicine, 67(4), 1106-1113.

Sheth VR, Liu G, Li Y, Pagel MD. (2012). Improved pH measurements with a single PARACEST MRI contrast agent. Contrast Media & Molecular Imaging, 7(1), 26-34.

Song X, Gilad AA, Joel S, Liu G, Bar-Shir A, Liang Y, Gorelik M, Pekar JJ, van Zijl PC, Bulte JW, McMahon MT. (2012). CEST phase mapping using a length and offset varied saturation (LOVARS) scheme. Magnetic Resonance in Medicine, 68(4), 1074-1086.

Liu G, Liang Y, Bar-Shir A, Chan KW, Galpoththawela CS, Bernard SM, Tse T, Yadav NN, Walczak P, McMahon MT, Bulte JW, van Zijl PC, Gilad AA. (2011). Monitoring enzyme activity using a diamagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent. Journal of the American Chemical Society, 133(41), 16326-16329.

Liu G, Gilad AA. (2011). MRI of CEST-based reporter gene. Methods in Molecular Biology, 771, 733-746.

Li Y, Sheth VR, Liu G, Pagel MD. (2011). A self-calibrating PARACEST MRI contrast agent that detects esterase enzyme activity. Contrast Media & Molecular Imaging, 6(4), 219-228.

Choi J, Kim K, Kim T, Liu G, Bar-Shir A, Hyeon T, McMahon MT, Bulte JW, Fisher JP, Gilad AA. (2011). Multimodal imaging of sustained drug release from 3-D poly(propylene fumarate) (PPF) scaffolds. Journal of Controlled Release, 156(2), 239-245.

Liu G, Bulte JW, Gilad AA. (2011). CEST MRI reporter genes. Methods in Molecular Biology, 711, 271-280.

Liu G, Gilad AA, Bulte JW, van Zijl PC, McMahon MT. (2010). High-throughput screening of chemical exchange saturation transfer MR contrast agents. Contrast Media & Molecular Imaging, 5(3), 162-170.

Ali MM, Liu G, Shah T, Flask CA, Pagel MD. (2009). Using two chemical exchange saturation transfer magnetic resonance imaging contrast agents for molecular imaging studies. Accounts of Chemical Research, 42(7), 915-924.

Liu G, Ali MM, Yoo B, Griswold MA, Tkach JA, Pagel MD. (2009). PARACEST MRI with improved temporal resolution. Magnetic Resonance in Medicine, 61(2), 399-402.

Liu G, Li Y, Pagel MD. (2007). Design and characterization of a new irreversible responsive PARACEST MRI contrast agent that detects nitric oxide. Magnetic Resonance in Medicine, 58(6), 1249-1256.

Ma Y, Liu G, Du M, Stayton I. (2004). Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis. Electrophoresis, 25(10-11), 1473-1484.

Liu G, Chen J, Ma Y. (2004). Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection. Journal of Chromatography. B, Analytical Techniques in Biomedical Life Sciences, 805(2), 281-288.

Chen J, Huang YW, Liu G, Afrasiabi Z, Sinn E, Padhye S, Ma Y. (2004). The cytotoxicity and mechanisms of 1,2-naphthoquinone thiosemicarbazone and its metal derivatives against MCF-7 human breast cancer cells. Toxicology and Applied Pharmacology, 197(1), 40-48.